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为了研究水稻Os CERK2基因的功能,构建了Os CERK2基因过表达和RNAi载体,利用农杆菌介导的遗传转化获得转基因植株,经多代筛选和分子检测,获得了4个超量表达的过表达纯系和2个转录水平下降的RNAi纯系。转基因植株经病原微生物细胞壁成分喷雾处理,采用半定量RT-PCR方法分析转基因植株中病程相关(PR)基因的表达情况,结果发现,诱导后过表达植株中PR基因表达增强,而RNAi植株中表达下降;抗病性鉴定结果表明,过表达转基因植株对白叶枯菌致病小种PXO99的抗性增强,而RNAi植株与野生型差异不显著。结果表明,过量表达Os CERK2基因可能是水稻抗病的有效途径。
In order to study the function of rice Os CERK2 gene, Os CERK2 gene overexpression and RNAi vector were constructed. Agrobacterium-mediated genetic transformation was used to obtain transgenic plants. After several generations of screening and molecular detection, overexpression of overexpression Pure lines and two RNAi lines with decreased transcript levels. Transgenic plants were spray-treated with the cell wall components of pathogenic microorganisms. Semi-quantitative RT-PCR was used to analyze the expression of disease-related (PR) genes in the transgenic plants. The results showed that the expression of PR gene was enhanced in the overexpressed plants but not in the RNAi plants Decreased. The results of disease resistance showed that the resistance of over-expressed transgenic plants to PXO99 was enhanced, but the difference between RNAi plants and wild type was not significant. The results showed that overexpression of Os CERK2 gene may be an effective way to resist disease in rice.