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目的:探讨岩柏草总黄酮对实验性肝纤维化大鼠的保护作用。方法:采用50%CCl4皮下注射8周,在造模同时分别灌胃15、30、60 mg/kg剂量岩柏草总黄酮混悬液,并以水飞蓟宾为实验对照。8周后处死大鼠,对大鼠肝脏纤维化评分,并检测血清和肝组织各指标。结果:CCl4造模结束后,大鼠肝组织病理结果显示其肝纤维化处于III-IV期;与模型组比较,岩柏草总黄酮能显著降低大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和肝组织中丙二醛(MDA)水平,明显升高肝组织中超氧化物歧化酶(SOD)水平,显著改善大鼠肝纤维化的病理形态。结论:岩柏草总黄酮对CCl4诱导的大鼠肝纤维化有较好的保护作用。
Objective: To investigate the protective effect of total flavonoids of kobuko on rats with experimental hepatic fibrosis. Methods: Fifty percent CCl4 was injected subcutaneously for 8 weeks. At the same time, 15, 30, and 60 mg / kg of total flavonoids of Kobuscritelium japonicus were instilled, respectively. After 8 weeks, the rats were sacrificed and the liver fibrosis scores of rats were determined. The indexes of serum and liver tissues were also measured. Results: The pathological results of rat liver tissue showed that the liver fibrosis was in stage III-IV after CCl4 modeling. Compared with the model group, the total flavonoids of A. japonica could significantly reduce the levels of serum ALT, AST, (AST) and malondialdehyde (MDA) in liver tissue, and significantly increased the level of superoxide dismutase (SOD) in liver tissue and significantly improved the pathological changes of hepatic fibrosis in rats. Conclusion: The total flavonoids of kobresia have good protective effect on CCl4-induced hepatic fibrosis in rats.