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目的 探讨钙、镁、铜、锌、维生素D和大豆联合干预对燃煤型氟中毒雌鼠氟斑牙、骨氟和尿氟的影响.方法 将120只SD雌鼠分为对照组、染氟组、高氟+钙尔奇牌添佳片组(即多种元素干预组)、高氟+钙尔奇牌添佳片+大豆组(即多种元素+大豆干预组),每组30只,动物自由食用不同配方的织金病区原煤加拌泥煤烘烤的玉米饲料,构建燃煤型氟中毒动物模型.每组分别于染氟60、120、180 d选择10只大鼠,动态观察雌鼠染氟期间氟斑牙情况,分别采用离子选择电极法、高温灰化-氟离子选择电极法测定雌鼠尿氟、骨氟含量.结果 随着染氟时间的延长,氟斑牙症状加重,干预组大鼠氟斑牙程度比染氟组轻.染氟组雌鼠尿氟、骨氟含量均高于同一时间点(60、120、180d)的对照组,差异有统计学意义(P＜0.05).同一时间点时,染氟组、多种元素干预组、多种元素+大豆干预组雌鼠的尿氟含量依次增加,差异均有统计学意义(P＜0.05);染氟组雌鼠骨氟含量高于对照组,但多种元素干预组、多种元素+大豆干预组骨氟含量均低于染氟组,差异有统计学意义(P＜0.05).随着染毒时间的延长,染氟组、多种元素干预组、多种元素+大豆干预组的尿氟含量均逐渐增加,差异有统计学意义(P＜0.05);染氟组骨氟含量逐渐增加,多种元素干预组、多种元素+大豆干预组骨氟含量逐渐降低,差异均有统计学意义(P＜0.05).结论 高剂量氟对雌鼠有明显毒性作用,多种营养素干预可拮抗其毒性作用,若在此基础上添加大豆联合干预则拮抗作用更明显.“,”Objective To investigate the effect of combined intervention of calcium,magnesium,copper,zinc,vitamin D and soybean on dental fluorosis,bone fluoride and urinary fluoride in female rats with coal-burning fluorosis.Methods A total of 120 SD female rats were randomly divided into the control group,the fluoride group,high fluoride+Caltrate Carbonate and Vitamin D Tablets group,as well as high fluoride + Caltrate Carbonate and Vitamin D Tablets + soybean group,30 rats in each group.The baked corn feed prepared by different formula of mixing raw coal and peat in the endemic area of Zhijin were given to the rats to establish the animal models of coal-burning fluorosis.Subsequently,10 rats were selecteded on the 60th,120th,and 180th day in each group,dynamic observation of dental fluorosis was performed in female rats exposed to fluoride,besides,bone fluoride and urinary fluoride were measured by using ion selective electrode method and high temperature ashing-ion selective electrode method,respectively.Results With prolonged exposure to fluoride,dental fluorosis symptoms aggravated and the degree of dental fluomsis in rats of the intervention group was slighter than that in the fluoride group.Furthermore,the content of bone fluoride and urinary fluoride in the fluoride group were higher than those in the control group at the same time point (on the 60th,120th,and 180th day),the differences were statistically significant (P＜0.05).Meanwhile,there were statistical differences that the urinary fluoride content of the female rats were increased successively at the same time point in the fluoride group,multi-nutrient intervention group and multi-nutrient + soybean intervention group (P＜0.05).The fluoride content in the fluoride group was higher than that in the control group,however,it was lower in the multi-nutrient intervention group and multi-nutrient+soybean intervention group than that in the fluoride group,the difference was statistically significant (P＜0.05).With the extension of the exposure time,the differences were statistically significant that gradual increase was found in the urinary fluoride content in the fluoride group,multi-nutrient intervention group and multi-nutrient + soybean intervention group (P＜0.05).In addition,statistical differences were found that the fluoride content was increased gradually in the fluoride group,but decreased in the multi-nutrient intervention group and multi-nutrient + soybean intervention group (P＜ 0.05).Conclusion High-dose fluoride has obvious toxic effects on female rats,multi-nutrient intervention can antagonize its toxicity and the antagonism is more obvious on the basis of adding soybean for combined intervention.