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目的:研究通关藤化学成分的指纹图谱。方法:采用HPLC以TC-C18色谱柱(4.6 mm×150 mm,5μm),乙腈-水为流动相进行梯度洗脱,检测波长210 nm,流速1.0 mL·min-1,柱温25℃。结果:以ZP-7通关藤苷元B([11α-O-(2-甲基丁酰基)-12β-O-乙酰基通关藤苷元B])为参照物,建立了通关藤的指纹图谱,指认了17个共有峰,精密度和重复性中共有峰相对保留时间RSD<3%,每个产地色谱图中的共有峰的峰面积之和>90%,10批药材的相似度在0.75~0.98,存在一定差异。结论:该方法准确、重复性好,为通关藤质量评价和质量控制提供依据,也为通关藤进一步开发奠定基础。
Objective: To study the fingerprints of chemical constituents of Pueraria lobata. Methods: The mobile phase was eluted with a gradient of acetonitrile and water on a TC-C18 column (4.6 mm × 150 mm, 5 μm). The detection wavelength was 210 nm and the flow rate was 1.0 mL · min-1. The column temperature was 25 ℃. Results: The fingerprinting of Cleveland avenae was established with reference to ZP-7 ettinide B ([11α-O- (2-methylbutyryl) -12β-O-acetyltrigitato element B] , 17 common peaks were identified, the RSDs of common peaks in precision and repeatability were less than 3% RSD, the sum of peak areas of common peaks in each chromatogram was> 90%, and the similarity of 10 batches of herbs was 0.75 ~ 0.98, there are some differences. Conclusion: The method is accurate and reproducible, and provides the basis for the quality evaluation and quality control of Viburnum vinifera. It also lays the foundation for the further development of Viburnum vines.