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目的 :为了探讨GST -π在食管癌发生、发展中的作用及其对食管癌早期诊断的意义。方法 :应用抗GST -π多克隆抗体 ,以石蜡切片LSAB免疫组化方法对 42例食管鳞状细胞癌及相应的癌旁组织进行检测。结果 :①GST -π在不典型增生、原位癌 ,Ⅰ级鳞癌的细胞核内高表达 ,明显高于正常粘膜。②在癌旁组织的底层中 ,仅原位癌的核表达阳性率与正常粘膜相比具有显著差异 (P <0 .0 1 ) ;在中层和表层 ,不典型增生和原位癌的GST -π核表达与正常和单纯性增生相比均有显著差异 (P <0 .0 2 5)。结论 :GST -π可能是食管癌发生的一个早期酶学标志物 ,对食管鳞癌的早期诊断具有重要意义
Objective: To investigate the role of GST-π in the occurrence and development of esophageal cancer and its significance in the early diagnosis of esophageal cancer. Methods: The anti-GST-π polyclonal antibody was used to detect 42 cases of esophageal squamous cell carcinoma and corresponding paracancerous tissues by paraffin section LSAB immunohistochemistry. Results: ①GST-π was highly expressed in the nucleus of atypical hyperplasia, carcinoma in situ and grade Ⅰ squamous cell carcinoma, which was significantly higher than that of normal mucosa. (2) In the paracancer tissues, the positive rate of nuclear expression in carcinoma only in situ was significantly higher than that in normal mucosa (P <0.01). In the middle and upper layers of atypical hyperplasia and carcinoma in situ, the GST - π nuclear expression compared with normal and simple hyperplasia were significantly different (P <0. 025). Conclusion: GST-π may be an early enzymatic marker of esophageal carcinogenesis, which is of great significance for the early diagnosis of esophageal squamous cell carcinoma