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目的:建立高效液相色谱法测定α-细辛脑及其制剂的含量。方法:色谱柱Diamonsil ODS-C18柱(200 mm×4．6 mm,5μm),流动相为甲醇-水(70:30),检测波长258 nm。结果:在20-100μg·ml-1浓度范围内呈良好的线性关系γ=0．999 8,平均回收率为99．8％,RSD=0．4％。结论:该法具有操作简便,快速,重现性好,结果准确的优点。
Objective: To establish a HPLC method for the determination of α-asarone and its preparations. METHODS: The chromatographic column Diamonsil ODS-C18 column (200 mm×4.6 mm, 5 μm) was used. The mobile phase was methanol-water (70:30) and the detection wavelength was 258 nm. RESULTS: There was a good linear relationship in the concentration range of 20-100 μg·ml-1 γ=0.999 8, the average recovery was 99.8%, and RSD was 0.4%. Conclusion: This method has the advantages of simple operation, rapidity, good reproducibility, and accurate results.