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目的研究脑挫伤后ARP-SRP基因表达谱差异及其法医学意义。方法从脑组织提取mRNA,应用ARP-SRP-1.0S基因芯片,研究脑挫伤组织与对照脑组织细胞凋亡蛋白和应激反应蛋白相关基因特异性表达差异。结果基因芯片杂交结果中,发现人类溶酶体的胃蛋白酶抑制剂不敏感蛋白酶基因(CLN2)表达水平显著下降。结论CLN2基因与一种婴儿致死性神经变性疾病LINCL有关,但CLN2基因在脑损伤中的作用尚有待进一步研究;基因芯片在研究脑损伤后相关基因的改变具有快速、高通量、高敏度等特点。
Objective To study the difference of ARP-SRP gene expression profile after brain contusion and its forensic significance. Methods mRNA was extracted from brain tissue and ARP-SRP-1.0S gene chip was used to study the specific expression of apoptosis-related proteins and stress-related proteins in brain contusion and control brain tissue. Results In the result of the hybridization of gene chips, it was found that the expression level of pepsin inhibitor-insensitive protease gene (CLN2) in human lysosomes was significantly decreased. Conclusions The CLN2 gene is associated with LINCL, a fatal neurodegenerative disease in infants. However, the role of CLN2 in brain injury remains to be further studied. The changes of related genes after gene chip research in brain injury are rapid, high-throughput and high-sensitivity Features.