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本研究分析了不同缺水条件下(缺水0 h,缺水2 h和缺水10 h)两个大豆品种(抗旱品种DT2008和栽培品种Williams82)的差异表达基因,并对差异表达基因进行了功能和通路富集分析。进一步通过整合大豆相关miRNA数据库,挖掘了miRNA与差异表达基因的调控网络。结果表明,在缺水2 h条件下抗旱品种DT2008表达相对较多的基因,差异基因数是Williams82的1.87倍;随着缺水时间由2 h增加到10 h,DT2008差异表达基因数没有显著变化,而Williams82差异表达基因增加了311个,表明干旱胁迫对于DT2008基因表达影响相对较小。差异表达基因主要富集到了apoptosis、cell death、response to stimulus及binding等生物学过程和分子功能,这些基因的差异表达降低了干旱胁迫诱导下的细胞凋亡并为干旱胁迫下大豆的生长发育提供了必需的营养。miRNA和mRNA调控网络分析结果表明,miR166家族、miR2118a-5p和miR2118b-5p能够调节Glyma13g23680.1和Glyma02g10320.1基因表达并参与大豆抗旱胁迫应答。
In this study, we analyzed the differentially expressed genes of two soybean cultivars (drought-tolerant cultivar DT2008 and cultivated cultivar Williams82) under different water deficits (water deficit for 0 h, water deficit for 2 h and water deficit for 10 h), and the differentially expressed genes Functional and pathway enrichment analysis. Through the integration of soybean related miRNA database, the miRNAs and the differentially expressed genes’ regulatory networks were discovered. The results showed that the number of differentially expressed genes in drought-tolerant DT2008 was 1.87 times of that in Williams82 at 2 h of water deficit. With the increase of water-deficit time from 2 h to 10 h, the number of differentially expressed genes in DT2008 did not change significantly , And Williams82 differential expressed genes increased by 311, indicating that drought stress has a relatively small effect on DT2008 gene expression. The differentially expressed genes are mainly enriched in the biological processes and molecular functions of apoptosis, cell death, response to stimulus and binding. The differential expression of these genes reduces the apoptosis induced by drought stress and provides the growth and development of soybean under drought stress The necessary nutrition. MiRNA and mRNA regulatory network analysis showed that miR166 family, miR2118a-5p and miR2118b-5p could regulate the expression of Glyma13g23680.1 and Glyma02g10320.1 genes and participate in the response to drought stress of soybean.