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目的 :探讨牛脑垂体提取物对维持角朊细胞生长的重要性。方法 :采用无血清培养技术 ,以添加各种生物因子的 MCDB1 53培养基为培养液 ,在体外培养成人包皮角朊细胞。结果 :在本实验条件下 ,牛脑垂体提取物是维持皮肤表皮角朊细胞增殖不可缺少的添加成分。结论 :牛脑垂体提取物是角朊细胞无血清培养的重要添加成分
Objective: To investigate the importance of bovine pituitary extract in maintaining keratinocyte growth. Methods: Serum-free cultured human adult keratinocytes were cultured in vitro with MCDB1 53 medium supplemented with various biological factors. Results: Under the experimental conditions, bovine pituitary extract is an indispensable additive to maintain the proliferation of keratinocytes in the skin. Conclusion: Bovine pituitary extract is an important additive component of keratinocyte serum-free culture