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目的 了解膀胱癌中H—ras基因突变和P21蛋白表达情况。方法 应用PCR—SSCP银染和免疫组化方法对31例膀胱癌组织分别进行检测。结果 发现H—ras基因突变发生率为38.7％,P21蛋白阳性率为71.0％。结论 二者存在一定的关联,并与临床分期、病理分级有密切联系。H—ras基因突变和P21蛋白表达可望作为膀胱癌诊断和判断预后有价值的指标。
Objective To investigate the mutation of H-ras gene and the expression of P21 in bladder cancer. Methods 31 cases of bladder cancer tissues were detected by PCR-SSCP silver staining and immunohistochemistry. The results showed that H-ras gene mutation rate was 38.7%, P21 protein positive rate was 71.0%. Conclusion There is a certain correlation between the two, and with clinical stage, pathological grade are closely linked. H-ras gene mutation and P21 protein expression is expected as a valuable indicator of bladder cancer diagnosis and prognosis.