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[Objective] To identify the characteristics of nrDNA-ITS from the leaf of famous-region drug Codonopsis tangshen Oliv.It will provide molecular evidence to distinguish C.tangshen Oliv.[Method] Direct PCR sequencing was used to detect the homology of nrDNA-ITS sequence of C.tangshen Oliv and analyze its sequence composition.[Result] The ratio of OD260/OD280 of genome DNA absorption value in samples extracted by improved CTAB was 1.8-2.0,DNA strips were clear and tidy,no obvious tail phenomenon,indicating that purity and quality of DNA samples was better,met the test requirements.NrDNA-ITS sequences universal primers were used to PCR amplification reactions,the expected double-stranded product of about 800 bp were obtained.After sequencing,it revealed that the Nucleotide sequence length of C.tangshen Oliv.nrDNA-ITS1 sequence was 260 bp,its G+C contents were 52%,the nucleotide sequence length of nrDNA-ITS sequence,and had been submitted to GenBank,registration No.was GQ906566.[Conclusion] DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of C.tangshen Oliv,which can also provide the basic information for molecular identification of C.tangshen Oliv.
[Objective] To identify the characteristics of nrDNA-ITS from the leaf of famous-region drug Codonopsis tangshen Oliv. It will provide molecular evidence to distinguish C. tangshen Oliv. [Method] Direct PCR sequencing was used to detect the homology of nrDNA- ITS sequence of C. tangshen Oliv and analyze its sequence composition. [Result] The ratio of OD260 / OD280 of genome DNA absorption value in samples extracted by improved CTAB was 1.8-2.0, DNA strips were clear and tidy, no obvious tail phenomenon, indicating that purity and quality of DNA samples was better, met the test requirements. NrDNA-ITS sequences universal primers were used to PCR amplification reactions, the expected double-stranded product of about 800 bp were obtained. After sequencing, it revealed that the Nucleotide sequence length of C. tangshen Oliv.nrDNA-ITS1 sequence was 260 bp, its G + C contents were 52%, the nucleotide sequence length of nrDNA-ITS sequence, and had been submitted to GenBank, registration No. was GQ906566. ] DNA sequencing can provide an accurate and reliable mean for the identification of the origin and genuineness of C. tangshen Oliv, which can also provide the basic information for molecular identification of C. tangshen Oliv.