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用RAPD技术对我国的34个辣(甜)椒品种进行了分析,22个随机引物共扩增出119条带,其中67条具多态性。采用Nei、Jaccard和欧氏距离3种方法计算各品种间的遗传距离矩阵,分别根据3个遗传距离矩阵进行UPGMA聚类分析,得出3个树形聚类图。聚类结果表明,IBPGR将辣椒种划分为4个变种的建议更为合理,Nei方法与Jaccard方法的结果均认为圆锥椒与灯笼椒的亲缘关系较近,而欧氏距离法的结果则认为所有辛辣类型的亲缘关系较近。研究还发现,栽培及育种活动在变种的发展过程中起到了重要作用。
34 varieties of spicy sweet pepper in China were analyzed by RAPD technique. A total of 119 bands were amplified by 22 random primers, of which 67 were polymorphic. Three kinds of methods of Nei, Jaccard and Euclidean distance were used to calculate the genetic distance matrix of each breed, UPGMA clustering analysis was conducted based on three genetic distance matrices respectively, and three tree clusters were obtained. The clustering results showed that it was more reasonable for IBPGR to classify the capsicum into four varieties. The results of Nei and Jaccard suggested that the genetic relationship between pepper and bell pepper is close, while the results of Euclidean distance are all Spicy type of close relationship. The study also found that cultivation and breeding activities played an important role in the development of varieties.