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目的:探讨蛋白酶体抑制剂对未分化甲状腺癌FRO细胞中过氧化物还原酶1(Prx1)表达的影响,以及Prx1在蛋白酶体抑制剂诱导FRO细胞凋亡中的作用。方法:选取人甲状腺未分化癌细胞系FRO,分别设培养液处理空白对照组,蛋白酶体抑制剂MG132、PSI、Lactacystin处理组;利用RNA干扰技术降低Prx1的表达;实时定量RT-PCR法、蛋白质印迹法分别检测各组细胞Prx1 mRNA及蛋白表达;流式细胞仪(FCM)检测细胞凋亡率。结果:与空白对照组相比,蛋白酶体抑制剂MG132、PSI和Lactacystin均显著增加FRO细胞系中Prx1基因表达水平(P<0.01);与空白对照组和随机序列核酸siRNA组相比,siPrx1处理组中Prx1 mRNA及蛋白表达水平显著降低(P<0.01),其凋亡率显著增加(P<0.01)。结论:蛋白酶体抑制剂能够上调甲状腺未分化癌FRO细胞系中Prx1基因的表达水平,siPrx1具有特异性降低Prx1基因表达的作用,同时增加蛋白酶体抑制剂诱导甲状腺癌FRO细胞凋亡作用。
AIM: To investigate the effect of proteasome inhibitor on the expression of peroxiredoxin 1 (Prx1) in undifferentiated thyroid carcinoma (FRO) cells and the role of Prx1 in proteasome inhibitor-induced FRO apoptosis. Methods: The human thyroid undifferentiated carcinoma cell line FRO was selected, and the culture medium was used to treat the blank control group, proteasome inhibitor MG132, PSI, Lactacystin treatment group respectively; RNA interference technology was used to reduce the expression of Prx1; The expression of Prx1 mRNA and protein were detected by Western blotting and flow cytometry (FCM). Results: Compared with the blank control group, the proteasome inhibitor MG132, PSI and Lactacystin significantly increased the Prx1 gene expression in FRO cell line (P <0.01). Compared with the blank control group and the random sequence siRNA group, siPrx1 treatment The expression of Prx1 mRNA and protein was significantly decreased (P <0.01), and the apoptosis rate was significantly increased (P <0.01). CONCLUSION: Proteasome inhibitor can up-regulate the expression of Prx1 in FRO cell line of thyroid undifferentiated carcinoma. SiPrx1 can specifically reduce the expression of Prx1 gene and increase the apoptosis of thyroid carcinoma FRO cells induced by proteasome inhibitor.