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目的观察白细胞介素(IL)-10和肿瘤坏死因子(TNF)-α在肾小球硬化模型大鼠中的表达,探讨其与肾小球硬化发生的关系。方法将20只SPF级雄性SD大鼠随机分为正常组(5只)、假手术组(5只)和模型组(10只)。模型组于大鼠右背行右肾切除术,术后第7天,尾静脉注射阿霉素(ADR)5 mg/kg,间隔21 d后再次注射ADR 3 mg/kg,建立大鼠肾小球硬化模型;假手术组剥离右肾包膜,尾静脉注射等剂量生理盐水;正常组仅尾静脉注射等剂量生理盐水。分别于术后1,3,5,7,9,11周检测24 h尿蛋白定量;于术后11周心脏采血处死大鼠,采用全自动生化分析仪检测血清尿素氮(BUN)、肌酐(Scr),并计算大鼠脾脏指数(SI)、胸腺指数(TI);采用ELISA法检测血清IL-10,TNF-α;HE染色观察肾脏病理改变,并计算肾小球硬化指数(GSI)。结果与正常组比较,假手术组各项指标均无明显变化,差异无统计学意义(P>0.05);与正常组和假手术组比较,模型组造模后大鼠体质量、SI、TI、血清IL-10表达量显著降低,24 h尿蛋白定量、GSI、血清BUN及Scr含量、血清TNF-α表达量显著升高,差异均有统计学意义(P<0.05)。病理结果显示,模型组肾小球呈局灶节段性硬化,间质内有明显淋巴细胞浸润,并有纤维化。结论大鼠肾小球硬化的发生与I L-10和TNF-α的异常表达有关。
Objective To observe the expression of interleukin (IL) -10 and tumor necrosis factor (TNF) -α in rats with glomerulosclerosis and to explore their relationship with glomerulosclerosis. Methods Twenty SPF male SD rats were randomly divided into normal group (n = 5), sham operation group (n = 5) and model group (n = 10). The rats in model group were right-sided right nephrectomy. On the 7th day after operation, adriamycin (ADR) 5 mg / kg was given to the caudal vein and ADR 3 mg / kg after 21 days. Ball sclerosis model; sham operation group peeled off the right renal capsule, tail vein injection of normal saline; normal group only the tail vein injection of normal saline. The levels of urinary protein in 24 h were detected at 1, 3, 5, 7, 9, and 11 weeks after operation respectively. The rats were sacrificed at 11 weeks after operation by blood sampling. The levels of serum urea nitrogen (BUN), creatinine Scr). The spleen index (SI) and thymus index (TI) were calculated. Serum IL-10 and TNF-α were detected by ELISA. The pathological changes of kidney were observed by HE staining. Glomerular sclerosis index (GSI) was calculated. Results Compared with the normal group, there was no significant change in the indexes in the sham operation group (P> 0.05). Compared with the normal group and sham operation group, the body weight of the model group, SI, TI , Serum IL-10 expression was significantly reduced, 24 h urinary protein, GSI, serum BUN and Scr levels, serum TNF-α expression was significantly increased, the difference was statistically significant (P <0.05). Pathological results showed that the model group showed focal segmental glomerular sclerosis, interstitial lymphoblastic infiltration, and fibrosis. Conclusion The occurrence of glomerulosclerosis in rats is related to the abnormal expression of IL-10 and TNF-α.