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目的采用四唑盐药敏试验(MTT)方法评价非小细胞肺癌(NSCLC)术后NP方案化疗的有效性,筛选敏感病例,指导NSCLC的个体化治疗。方法共收集144例病理证实为ⅠB～ⅢA期NSCLC患者的临床资料,术后均接受3～4个周期的NP方案化疗,且随访数据完整。MTT法检测各病例的肿瘤组织对顺铂(DDP)及长春瑞滨(NVB)的药物敏感性。结果①体外药敏试验的结果与临床治疗疗效的总体符合率为76.4%,体外MTT药敏试验的敏感度为87.4%,特异度为55.1%。②鳞癌体外药敏试验的结果与临床治疗疗效的总体符合率为85.3%,敏感度为92.6%,特异度为57.1%;腺癌体外药敏试验的结果与临床治疗疗效的总体符合率为62.7%,敏感度为86.2%,特异度为31.8%;腺鳞混合型体外药敏试验的结果与临床治疗疗效的总体符合率为78.3%,敏感度为66.7%,特异度为90.9%。结论①体外药敏试验与临床治疗敏感性有很好的相关性,是指导临床化疗的一种可行而又重要药物筛选方法。②体外药敏试验对非小细胞肺癌患者术后选择NP方案化疗提供了依据。
Objective To evaluate the efficacy of NP regimen chemotherapy in patients with non-small cell lung cancer (NSCLC) by the tetrazolium resistance test (MTT) method. Screen the sensitive cases and guide the individualized treatment of NSCLC. Methods The clinical data of 144 pathologically confirmed NSCLC patients with stage ⅠB ~ ⅢA were collected. All the patients received NP chemotherapy three to four cycles postoperatively, and the follow - up data were complete. The MTT assay was used to detect the drug sensitivity of tumor tissues to cisplatin (DDP) and vinorelbine (NVB). Results ① The results of in vitro susceptibility test and clinical curative effect were 76.4%. The sensitivity of in vitro MTT assay was 87.4% and the specificity was 55.1%. ② The results of in vitro susceptibility testing of squamous cell carcinoma and clinical curative effect were 85.3%, 92.6% and 57.1%, respectively. The overall in-vitro coincidence rate of results of in vitro susceptibility test and clinical curative effect of adenocarcinoma was The sensitivity and specificity were 86.7%, 62.7%, 86.2% and 31.8%, respectively. The in-vitro susceptibility test of adenosquamous mixed with the clinical curative effect was 78.3%, the sensitivity was 66.7% and the specificity was 90.9%. Conclusion ① In vitro susceptibility testing has a good correlation with clinical treatment sensitivity and is a feasible and important drug screening method to guide clinical chemotherapy. ② In vitro susceptibility test for non-small cell lung cancer patients choose NP regimen provided the basis for chemotherapy.