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酶法脱毛是制革及纺织工业清洁化生产的关键环节,为提高枯草工程菌株WB800N/p HT43-npr产脱毛用中性蛋白酶(JW-3脱毛酶)的发酵水平,在单因素试验的基础上,采用4因素3水平的响应面分析法,对产酶培养基进行优化,同时对酶法回收制革中废弃的兔尾绒毛进行研究。结果表明工程菌株产酶的最佳培养基为:玉米粉23.00 g/L,蛋白胨26.00 g/L,Mn SO42.10 mg/L,Ca Cl20.13 g/L,在37℃,200 r/min的培养条件下,蛋白酶发酵活力可达990.12 U/m L。对兔尾脱毛试验表明JW-3脱毛酶能够全部脱掉兔尾绒毛,脱毛总用时较1398、2709蛋白酶短;JW-3脱毛酶处理兔毛纤维的显微结构与对照无明显区别,且能增加兔毛纤维鳞片的摩擦系数,从而降低兔绒织品的掉毛率。本研究为纺织工业中动物毛发的清洁化生产及制革工业废弃动物毛发的再利用提供了技术参考。
Enzymatic hair removal is a key step in tannery and textile industry clean production. In order to improve the fermentation level of WB800N / p HT43-npr hair removal neutral protease (JW-3 hair removal enzyme), based on the single factor test The reaction medium of 4 factors and 3 levels was used to optimize the enzyme-producing medium. At the same time, the enzymatic method was used to recover the waste rabbit tail villus in tannery. The results showed that the optimal culture medium for the enzyme production was 23.00 g / L cornstarch, 26.00 g / L peptone, 42.10 mg / L MnSO4 and 20.13 g / L CaCl 2 at 37 ℃, 200 r / min Under the culture conditions, the protease fermentation activity up to 990.12 U / m L. The hair removal of rabbit tail showed that JW-3 hair removal enzyme could completely remove the tail hair of rabbit and hair removal was shorter than that of 1398,2709 protease. The microstructure of rabbit hair fiber treated by JW-3 hair removal enzyme had no significant difference with the control Increase the friction coefficient of rabbit hair fiber scales, thereby reducing the rabbit hair velvet fabric lint rate. This study provides a technical reference for the clean production of animal hair in the textile industry and the reuse of the fur of industrial animal waste.