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本研究旨在检测维生素E琥珀酸酯(VES)联合化疗药物对大肠癌细胞的凋亡诱导作用,为临床联合用药提供依据。一、材料和方法1.细胞和主要试剂:人大肠癌细胞株LS174T购自中科院上海细胞所,VES购自Sigma公司,噻唑蓝(MTT)比色法为Amresco产品,PE标记抗人Fas抗体购自晶美公司。2.细胞
The aim of this study was to investigate the apoptosis-inducing effect of vitamin E succinate (VES) and chemotherapeutic agents on colorectal cancer cells and to provide a basis for clinical combination therapy. Materials and Methods 1. Cells and Major Reagents: Human colorectal cancer cell line LS174T was purchased from Shanghai Institute of Cell, Chinese Academy of Sciences, VES was purchased from Sigma, MTT colorimetric method was Amresco product, PE labeled anti-human Fas antibody purchased Since the United States crystal company. 2. Cells