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A new colorimetric and fluorescent probe,2-(2,4-dinitrostyryl)-1,3,3-trimethyl-3H-indolium iodide (DTI),for selective and sensitive detection of biological thiols is reported.In aqueous solution at physiological pH 7.4,biological thiols react with DTI via Michael addition to give the brownish red adduct concomitant with fluorescence emission decrease.
A new colorimetric and fluorescent probe, 2- (2,4-dinitrostyryl) -1,3,3-trimethyl-3H-indolium iodide (DTI), for selective and sensitive detection of biological thiols is reported. 7.4, biological thiols react with DTI via Michael addition to give the brownish red adduct concomitant with fluorescence emission decrease.