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目的:建立HPLC测定清热解毒胶囊中绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素含量的方法。方法:采用Eclipse XDB色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.4%磷酸溶液,梯度洗脱,流速0.80 m L·min-1,检测波长275nm。绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素与其相邻质谱峰能完全分离。结果:绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素的线性范围分别为7.83~313 mg·L-1(r=0.999 9),4.30~132 mg·L-1(r=0.999 8),0.836~33.5 mg·L-1(r=0.999 7),4.36~349 mg·L-1(r=1.000 0),0.731~58.4 mg·L-1(r=0.999 7),0.314~12.6 mg·L-1(r=0.999 7)。方法回收率均不低于98%。结论:该方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。
Objective: To establish a method for the determination of chlorogenic acid, geniposide, luteolin, baicalin, forsythin and wogonin in Qingrejiedu capsule by HPLC. Methods: Eclipse XDB column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid solution with a gradient elution at a flow rate of 0.80 m L · min-1. The detection wavelength was 275 nm. Chlorogenic acid, geniposide, luteolin, baicalin, forsythin and wogonin were completely separated from their adjacent peaks. Results: The linear ranges of chlorogenic acid, geniposide, luteolin, baicalin, forsythin and wogonin were 7.83-313 mg · L-1 (r = 0.999 9) and 4.30-132 mg · L (R = 0.999 8), 0.836-33.5 mg · L -1 (r = 0.999 7), 4.36-349 mg · L -1 (r = 1.000 0), 0.731-58.4 mg · L -1 (r = 0.999 7), 0.314 ~ 12.6 mg · L -1 (r = 0.999 7). Method recovery rate of not less than 98%. Conclusion: The method is simple, accurate, reproducible and can eliminate the interference of other components and can be used to evaluate the quality control of the preparation.