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目的 观察一氧化氮 (NO)和白细胞浸润对急性局灶性脑缺血再灌流损伤的作用。方法 建立大鼠急性大脑中动脉栓塞 (MCAO)及再灌流模型 ,采用NADPH -d组织化学方法检测大脑皮质神经元内NOS活性 ,利用MPO活性测定白细胞浸润。结果 ①在脑缺血 15min、30min再灌流 4h时 ,NOS、MPO活性均随着脑缺血时间延长而增加 (P<0 0 1) ;②在脑缺血 6 0min再灌流 4h时 ,NOS活性明显降低 (P <0 0 5 ) ,MPO活性显著增加 (P <0 0 5 )。结论 实验结果说明NO与白细胞浸润在急性脑缺血早期共同作用脑缺血 再灌流损伤 ;在急性脑缺血 再灌流后期 ,脑缺血再灌流损伤主要与白细胞浸润有关
Objective To observe the effects of nitric oxide (NO) and leukocyte infiltration on acute focal cerebral ischemia-reperfusion injury. Methods A rat model of middle cerebral artery occlusion (MCAO) and reperfusion was established. NADPH-d histochemical method was used to detect the activity of NOS in cerebral cortex neurons. The leukocyte infiltration was measured by MPO activity. Results ① The activity of NOS and MPO increased with the prolongation of cerebral ischemia (P <0.01) at 15 min after ischemia and 4 h after reperfusion at 30 min; ② At 4 h after reperfusion at 60 min after cerebral ischemia, NOS activity (P <0.05), MPO activity was significantly increased (P <0 05). Conclusions The experimental results show that NO and leukocyte infiltration play a role in cerebral ischemia-reperfusion injury in the early stage of acute cerebral ischemia. In the late stage of acute cerebral ischemia and reperfusion, the injury of cerebral ischemia and reperfusion is mainly related to leukocyte infiltration