Determination of reactive oxygen generated from natural medicines and their antibacterial activity

来源 :Journal of Pharmaceutical Analysis | 被引量 : 0次 | 上传用户:gzh0791
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Extracts of 16 natural medicine powders {Galla chinensis,Malloti cortex,Cassiae semen,Sophorae radix,Myricae cortex,Crataegi fructus,Gambit,Mume fructus,Geranii herba,Phellodendri cortex,Coptidis rhizoma,Swertiae herba,and Cinnamomi cortex) were assayed for reactive oxygen concentrations using the peroxyoxalate chemiluminescent detection system.High luminescence intensity was observed in Galla chinensis,Geranii herba,Malloti cortex,Myricae cortex,and Cinnamomi cortex.Additional experiments identified the reactive oxygen species as hydrogen peroxide.Galla chinensis generated 2.4 × 10~(-4) mol/L hydrogen peroxide from a 1 mg/mL solution.In bacterial growth tests,Galla chinensis extract had antibacterial activity against Escherichia coli.Staphylococcus aureus,Bacteroides thetaiotaomicron,Campylobacter sputorum biovar sputorum.Streptococcus salivarius thermophilus,Lactobacillus casei,and Bifidobacterium longum infantis.This antibacterial activity was decreased by the addition of catalase.It revealed that hydrogen peroxide which Galla chinensis produced participated in antibacterial activity. Extracts of 16 natural medicine powders {Galla chinensis, Malloti cortex, Cassiae semen, Sophorae radix, Myricae cortex, Crataegi fructus, Gambit, Mume fructus, Geranii herba, Phellodendri cortex, Coptidis rhizoma, Swertiae herba, and Cinnamomi cortex) were assayed for reactive oxygen concentrations using the peroxyoxalate chemiluminescent detection system. High luminescence intensity was observed in Galla chinensis, Geranii herba, Malloti cortex, Myricae cortex, and Cinnamomi cortex. Additional experiments identified the reactive oxygen species as hydrogen peroxide. Galla chinensis generated 2.4 × 10 ~ ( -4) mol / L hydrogen peroxide from a 1 mg / mL solution. In bacterial growth tests, Galla chinensis extract had antibacterial activity against Escherichia coli. Bifidobacterium longum infantis. This antibacterial activity was decreased by the addition of catalase. It revealed that hydrogen peroxide which Galla chinensis produced participated in antibacterial activity.
其他文献
那年,他才刚刚八岁,还是一个懵懂无知的孩子。每天带着弟弟和妹妹在外面疯玩,脸上渗出细细的汗珠,还有斑斑点点的污渍。那真是个少年不识愁滋味的年龄。  有大人问他,你多大了?他抬起头,眨着长长睫毛的眼睛,稚声稚气地回答,我还小呢,才八岁。大人抚摸着他的小脑袋瓜子,笑嘻嘻道,嗯,是还小,快快长大吧!他听了,嘿嘿一笑,一转身,就带着弟弟妹妹跑开了。  日子就这样一天一天流逝,像风儿刮过面颊,无声无息的。有
Objective: To determine antiacanthamoebic activity of natural and marketed honey samples.Methods: Natural honey samples were collected directly from the bee hiv
本文运用建构主义教育理论,结合课程本身特点,对数字信号处理课程进行教学方法和教学模式的探索和实践。通过转换教学主体、重现问题情景、激发兴趣、调整考核方式等手段,引
对于路桥建设工程来说,混凝土施工是整个施工过程中最为关键的环节,同时也是难度最大的环节。因此,要想提高路桥工程的施工技术,混凝土施工技术就是关键。由于普通混凝土普遍
看着小巧的梅赛德斯-奔驰A180,“大奔”两字实在难以说出口,可它真真是梅赛德斯-奔驰的嫡系。从五六米长的“大奔”S级,到E级、C级、B级,一直到眼前不足4米的A级,是缩微还是
一、光谱辐射度计量简介物体发出辐射主要有两种形式:一种是热辐射;另一种是光辐射。任何物体都会发射热辐射。温度低的物体,发出看不见的红外辐射,随着温度的升高,物体发出
曾有很多次见过飞度改装的案例,特别是去年,随着飞度的热销,很多激情的车主对其进行了音响升级,得美乐相随,飞度的动感特色发挥得随心所欲。虽说发烧友改装案例接触不少,但当
《风流一代》杂志是由共青团江苏省委主管主办的综合性青年刊物,1953年由《苏北青年》和《苏南农村青年报》合并而成,1985年改名为《风流一代》,运营至今已有60多年的办刊历
幽默逗乐是长寿之道。但也有一些人,他们狡猾、爱钻空子,可以将责任推卸得一干二净,也可以让你无言以对、啼笑皆非。他们的这些黑色幽默大多建立在伤害他人的基础上。最终,也
【摘要】由于税收执法体制改革的不断深入,我国的税收管理正朝着规范化、科学化的方向发展。现阶段由于税收征收体制不健全、税收人员的执法水平参差不齐、纳税人的法律意识逐渐增强等多方面因素的影响,税收执法风险不断地加大,影响了国家的财政税收。因此,本文从税收执法风险的概念入手,分析了税收执法风险产生的原因,然后提出了防范税收执法风险的对策。  【关键词】税收执法 风险表现 风险管理 防范对策  我国的财政