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以中薯3号脱毒微型薯为外植体,利用根瘤农杆菌介导ODREB2B基因转化马铃薯,经PCR和RT-PCR检测有6株为转基因植株,确定ODREB2B基因已整合到马铃薯的基因组中并正常转录和翻译。在0.5%NaCl和10%PEG胁迫处理1周后,马铃薯叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均呈现升高的趋势,但转基因植株升高幅度大于对照,二者差异达到极显著水平(P<0.01)。结果表明,ODREB2B基因的表达提高了中薯3号的抗干旱和耐盐能力。
In order to transform potato with ODREB2B gene mediated by Agrobacterium tumefaciens, potato transformed with microtuber-free potato tuber No. 3 was used as explant. Six transgenic plants were detected by PCR and RT-PCR. The ODREB2B gene was integrated into the genome of potato Normal transcription and translation. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in potato leaves showed an increasing trend after 0.5% NaCl and 10% PEG stress for 1 week, but the transgene Plants increased more than the control, the difference between the two reached a significant level (P <0.01). The results showed that the expression of ODREB2B gene increased the resistance to drought and salt of Zhongshu 3.