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目的:观察复方苯妥英钠凝胶剂对实验性创面修复及细菌感染的影响。方法:建立小鼠皮肤切割伤模型,用复方苯妥英钠凝胶剂低、高剂量(4.25,4.55 g.kg-1)于创面涂药,连续12 d,分别于3,6,8,10,12 d观察小鼠皮肤创面的收缩率;建立大鼠皮肤切割伴金黄色葡萄球菌感染模型,用复方苯妥英钠凝胶剂低、高剂量(1.70,1.82 g.kg-1)于创面涂药,连续12 d,观察大鼠皮肤创面的愈合率,同时对愈合组织进行病理学组织学检查。结果:3,6,8,10,12 d,复方苯妥英钠凝胶剂低、高剂量组小鼠创面收缩率分别为(20.16±5.78)%,(40.02±9.79)%,(71.04±7.27)%,(96.14±2.53)%,(99.44±1.30)%,(28.50±4.77)%,(55.66±8.39)%,(79.67±5.11)%,(96.14±2.53)%,(100.00±0.00)%,明显高于凝胶基质组(16.77±4.79)%,(29.95±5.63)%,(50.69±4.19)%,(67.47±5.13)%,(82.48±4.40)%(P<0.05,P<0.01,P<0.01,P<0.01;P<0.01,P<0.01,P<0.01,P<0.01,P<0.01);复方苯妥英钠凝胶剂低、高剂量组大鼠皮肤创面的愈合率分别为(29.45±5.11)%,(50.17±4.29)%,(72.48±5.05)%,(87.44±4.11)%,(96.66±3.75)%;(33.71±3.43)%,(58.67±5.59)%,(81.86±4.15)%,(95.92±2.39)%,(100.00±0.00)%明显高于凝胶基质组(20.71±3.58)%,(40.42±3.89)%,(58.53±6.21)%,(74.71±9.23)%,(86.47±7.15)%(均P<0.01)。结论:复方苯妥英钠凝胶剂能促进实验性细菌感染创面的愈合。
Objective: To observe the effect of compound phenytoin sodium gel on experimental wound repair and bacterial infection. Methods: The model of skin incised wounds in mice was established. The wounds were treated with compound phenytoin sodium low and high dosage (4.25,4.55 g.kg-1) for 12 days, respectively, at 3, 6, 8, 12 days later, the skin wound healing rate of mice was observed. The model of cutaneous wound healing in rats was established. Staphylococcus aureus infection was induced by compound phenytoin sodium gel in low and high dose (1.70,1.82 g.kg-1) For 12 consecutive days, the wound healing rate of the rat skin was observed, and the histopathological examination of the healing tissue was performed. Results: At 3, 6, 8, 10, 12 d, the contractile rates of wounds of mice treated with low dose and high dose of phenytoin sodium gel were (20.16 ± 5.78)%, (40.02 ± 9.79)% and (71.04 ± 7.27) %, (96.14 ± 2.53)%, (99.44 ± 1.30)%, (28.50 ± 4.77)%, (55.66 ± 8.39)%, (79.67 ± 5.11)%, (96.14 ± 2.53)%, (100.00 ± 0.00)%, , Which was significantly higher than that of the gel matrix group (16.77 ± 4.79)%, (29.95 ± 5.63)%, (50.69 ± 4.19)%, (67.47 ± 5.13)% and (82.48 ± 4.40)% , P <0.01, P <0.01; P <0.01, P <0.01, P <0.01, P <0.01, P <0.01). The healing rates of the compound phenytoin sodium gel low and high dose groups were (29.45 ± 5.11)%, (50.17 ± 4.29)%, (72.48 ± 5.05)%, (87.44 ± 4.11)%, (96.66 ± 3.75)%, (33.71 ± 3.43)%, (58.67 ± 5.59)%, 81.86 ± 4.15%, 95.92 ± 2.39% and 100.00 ± 0.00% were significantly higher than that of the gel matrix group (20.71 ± 3.58)%, (40.42 ± 3.89)%, (58.53 ± 6.21)% and (74.71 ± 9.23)%, (86.47 ± 7.15)% (all P <0.01). Conclusion: Compound phenytoin sodium gel can promote wound healing of experimental bacterial infection.