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我们利用逆转录-聚合酶反应(RT-PCR)法,对49例乳腺癌组织进行了蛋白激酶A的Ⅰ型α调节亚单位(PKARⅠα)mRNA水平的检测并研究其与临床病理的关系。 一、材料和方法 1.标本来源:标本来自中国医学科学院肿瘤医院2001年9月至2002年9月间行单一外科手术治疗的乳腺癌患者
Using reverse transcription polymerase chain reaction (RT-PCR), we detected the level of PKARⅠα mRNA in 49 cases of breast cancer and studied its relationship with clinical pathology. First, the material and methods 1 specimen source: specimens from the Chinese Academy of Medical Sciences Cancer Hospital from September 2001 to September 2002 underwent a single surgical treatment of breast cancer patients