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在Hm—毒素——根冠细胞测定法中,处理的温度,离体根冠细胞的浓度,毒素制剂的pH值和光照等都不同程度地影响玉米根冠细胞的存活和Hm—毒素诱致根冠细胞死亡的结果。试验证明:在低温,有光照和偏酸性的基质下获得的测定结果不理想,而黑暗25℃下处理12个小时,离体根冠细胞浓度为1000个/ml,毒素制剂的pH值为7.0左右时测定最宜。试验还发现,虽然改良Fries培养基中的酒石酸铵,NH_4NO_3和NaCl诸组分会影响玉米根冠细胞的存活,但用这种培养基制备的培养物滤液诱致的玉米根冠细胞死亡仍比病叶提取液强。此外,不同玉米自交系的根冠细胞其自然存活率的差异也很大;测定的时间须控制在12小时以内。可见,进行定量的Hm—毒素生物测定,严格地控制上述条件是非常重要的。
In the Hm-toxin-root and crown cell assay, the temperature of treatment, the concentration of ex vivo root canals, the pH value of the toxin preparations and the illumination all affected the survival of root cap cells and Hm-toxin induced roots Results of crown cell death. Tests showed that the results obtained under low temperature, light and acidic substrate were not ideal, while the dark at 25 ℃ for 12 hours, the concentration of isolated root canopy cells was 1000 / ml, the pH value of the toxin preparation was 7.0 When measured around the most appropriate. It was also found that although ammonium tartrate, NH_4NO_3 and NaCl components in modified Fries medium affected the survival of corn root-cap cells, the death of corn root-cap cells induced by the culture filtrate prepared by this medium was still lower than that of diseased leaves Strong extract. In addition, rootstock cells of different maize inbred lines also have a large difference in their natural survival rate; the measurement time must be controlled within 12 hours. Visible, quantitative Hm-toxin bioassay, strict control of the above conditions is very important.