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本文采用琼脂糖电泳法测定血清磷酸肌酸激酶大分子同工酶－２（ＣｒｅａｔｉｎｅＫｉｎａｓｅｉｓｏｅｎｚｙｍｅ２，ＣＫ－Ｍ２）和酶免疫双抗体夹心法测定可溶性寡糖蛋白（ＯｌｉｇｏＭａｃｒｏｓａｃｃｈａｒｉｄｅＰｒｏｔｅｉｎ，ＯＰ）含量。经５７例胃癌、５１例胃癌前期病变（慢性萎缩性胃炎１９例，慢性萎缩性胃炎伴肠化１７例，残胃炎１１例，不典型增生４例）和２８例正常献血员血清测定。结果表明正常人血清ＣＫ－Ｍ２均为阴性。胃癌检出阳性率为５６１％，在胃部疾患中特异性为９８６３％，ＯＰ在胃癌组明显高于其他两组（Ｐ＜００５）。但ＣＫ－Ｍ２、ＯＰ与胃癌细胞类型及分化程度无关。提示ＣＫ－Ｍ２可作为胃癌的辅助诊断指标，尤其在胃部疾患的鉴别诊断上具有一定的意义；ＯＰ可作为评估胃癌患者体内免疫活性的指标。
In this study, the content of soluble oligosaccharide (OligoMacrosaccharideProtein, OP) was determined by agarose gel electrophoresis with serum creatine kinase kinases 2 (CK-M2) and enzyme-linked immunosorbent assay (ELISA) Fifty-seven cases of gastric cancer, 51 cases of gastric precancerous lesions (19 cases of chronic atrophic gastritis, 17 cases of chronic atrophic gastritis with intestinal metaplasia, remnant gastritis in 11 cases, atypical hyperplasia in 4 cases) and 28 normal blood donors serum determination. The results showed that normal serum CK-M2 were negative. The positive rate of gastric cancer was 561%, specificity of gastric disease was 9863%, OP was significantly higher in gastric cancer group than the other two groups (P <005). However, CK-M2, OP and gastric cancer cell type and degree of differentiation has nothing to do. These results suggest that CK-M2 can be used as an auxiliary diagnostic indicator of gastric cancer, especially in the differential diagnosis of gastric disorders. OP can be used as an index to evaluate the immune activity in gastric cancer patients.