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目的 :研究甘氨酸 (Gly)和多粘菌素B(PMB)联合应用对内毒素 (ET)的拮抗作用以及两者联用的毒性。方法 :(1)利用封闭群新西兰家兔发热模型 ,观察Gly和PMB联用对ET致热性的影响及其最佳组合比例 ,观察指标包括平均体温反应曲线、最大体温反应幅度、体温反应指数 ;(2 )利用LD50 观察Gly、PMB及两者联用对小鼠的毒性。结果 :(1)PMB、Gly对ET致热性有明显协同拮抗作用 (P <0 0 5 ) ;(2 )两者联合拮抗内毒素 (0 0 1μg)的最佳组合为PMB(30 0 0 0U) +Gly(15mg) ;(3)小鼠静脉给药PMB的LD50 为 6 0 6× 10 4 U/kg·w ,合剂 (组成为PMB30 0 0 0U∶Gly 15mg)中PMB的LD50 为 8 38× 10 4 U/kg·w ,剂量显著增大 (P <0 0 5 ) ,毒性明显下降。结论 :甘氨酸和多粘菌素B联合应用是增效降毒的。
Objective: To investigate the antagonistic effect of glycine (Gly) and polymyxin B (PMB) combined with endotoxin (ET) and their toxicity. Methods: (1) To investigate the effect of Gly and PMB on pyrethroidism and the optimal combination ratio of rabbits in a closed-feather-New Zealand rabbits model. The observed indexes include mean body temperature response, maximum body temperature response, body temperature response index ; (2) LD50 was used to observe the toxicity of Gly, PMB and their combination on mice. Results: (1) PMB and Gly had a synergistic antagonistic effect on the pyrogenic activity of ET (P <0.05). (2) The optimal combination of the combination of PMB and Gly with endotoxin (0 0 1 μg) was PMB (3) mice given intravenous PMB with an LD50 of 6 0 6 × 10 4 U / kg · w and PMB with a composition of PMB 30 0 0 0 U: Gly 15 mg had an LD50 of 8 38 × 10 4 U / kg · w, the dose was significantly increased (P <0 0 5), the toxicity decreased significantly. Conclusion: The combination of glycine and polymyxin B is synergistic and toxic.