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目的通过血袋表面细菌学调查,了解医院回收血袋的污染情况。方法采用《医院消毒卫生标准》(GB15982-1995)规定方法,对血袋表面取样进行细菌培养,鉴定采用ATB Expression系统配合手工方法。结果经统计学处理,血袋表面细菌污染率差异均有统计学意义(P<0.05),未干预组污染率(26.83%),干预组污染率(9.77%),库存血袋组污染率(0.49%)。细菌检出类别分别是G+球菌(29.58%),G-杆菌(38.03%),G+杆菌(22.54%)。未干预组和干预组检出致病菌金黄色葡萄球菌以及铜绿假单胞菌、大肠埃希氏菌、肺炎克雷伯氏菌等条件致病菌。结论血袋出库后污染情况加重,血袋统一回收到血库极易造成细菌2次污染及院内的交叉感染。
Objective Through the bacteriological investigation of the blood bag surface, understand the hospital recovery of blood bag pollution. Methods The method of “Sanitary Standard for Hospital Disinfection” (GB15982-1995) was adopted to carry out bacterial culture on the surface of blood bag samples. The method of ATB Expression system and manual method was used for identification. Results The statistical analysis showed that there was significant difference in the bacterial contamination rate on the surface of the blood bag (P <0.05), the contamination rate in the non-intervention group (26.83%), the contamination rate in the intervention group (9.77%), 0.49%). The types of bacteria detected were G + (29.58%), G- (38.03%) and G + (22.54%) respectively. Pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae were detected in the non-intervention group and intervention group. Conclusion The blood bag excreted after the pollution situation aggravating, blood bags unified recovery to the blood bank can easily lead to bacterial secondary pollution and hospital cross-infection.