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联合应用薄层色谱、紫外光谱测定小鼠体内安定。样品制备是在组织匀浆中加入磷酸二氢钠缓冲液（３ｍｏｌ／Ｌ），以乙醚振荡提取，离心分离。利用值、紫外吸收峰与谷值、吸收度比等进行安定的特征检识。薄层色谱条件：硅胶Ｇ板，分别用苯－丙酮（３：２）及丙酮－苯－氯仿（２．５：４．０：５．０）作为展开剂。定量分析结果表明以Ａ值为定量参数较之以上Ａ值为定量参数有更高的灵敏度；而测定值的偏倚随样品浓度增大而变小；不同介质和不同浓度水平下的回收率不同（６０～９０％）。小鼠口服安定１ｈ后肝和脑中可明显检出安定，但胃中的安定浓度高得多。
Combined application of thin layer chromatography, UV spectrophotometry in vivo stability in mice. Samples were prepared by adding sodium dihydrogen phosphate buffer (3mol / L) to the tissue homogenate, extracted with ether and centrifuged. Using the value of UV absorption peak and valley, absorption ratio stability characteristics of the identification. TLC conditions: silica gel G plate with benzene-acetone (3: 2) and acetone-benzene-chloroform (2.5: 4.0: 5.0) as developing solvent. The results of quantitative analysis showed that the A value was more sensitive than the A value above, while the bias of the measured value was smaller with the increase of the sample concentration. The recoveries were different under different media and different concentration levels 60 to 90%). Oral stability of mice 1h after the liver and brain can be detected diazepam, but the stability of the stomach was much higher concentrations.