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目的评价红丝线草的亚慢性毒性,并初步研究其抗氧化功能。方法采用大鼠90 d喂养试验对红丝线草的亚慢性毒性进行评价,并在试验结束时,检测大鼠血清和肝组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果红丝线草的大鼠90 d喂养实验各项检测结果均未见异常,其最大未观察到有害作用剂量(NOAEL)大于10 000 mg/kg.BW。雌、雄大鼠血清和肝组织的SOD、GSH-Px水平均高于对照组,且至少有1个剂量组与对照组的差异有统计学意义(P<0.05),雌、雄各剂量组大鼠的血清MDA含量与对照组比较差异无统计学意义(P>0.05)。结论红丝线草在本实验条件下未见有亚慢性毒性,抗氧化指标检测结果提示红丝线草具有一定的抗氧化功能。
Objective To evaluate the subchronic toxicity of Rhododendron simiarum and to study its antioxidative function. Methods The subchronic toxicity of red thread grass was evaluated by 90-day feeding experiment in rats. At the end of the experiment, the levels of superoxide dismutase (SOD) and glutathione peroxidase GSH-Px) activity and malondialdehyde (MDA) content. Results The results of 90 days feeding experiment showed no abnormality in rats. The maximum no-effect dose (NOAEL) of more than 10 000 mg / kg.BW was observed. The levels of SOD and GSH-Px in serum and liver of female and male rats were higher than those in control group, and there was a significant difference between at least one dose group and control group (P <0.05) There was no significant difference in MDA level between the two groups (P> 0.05). Conclusion There is no sub-chronic toxicity in this experimental condition. The results of antioxidant test indicate that the red silk weed has certain anti-oxidant function.