Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells

• 作 者: Yuxin Wu Jinghan Zhang Xiaoming Ben
• 机 构: Department of Neurosurgery,Nanjing Children\'s Hospital of Nanjing Medical University,Department o
• 出 处: Neural Regeneration Research
• 发表日期: 2004年期
• 关键词: galactosidase differentiate neuronal colony cultured staining labeled expanded m

Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were separated and cultured using the “pour-off” method.Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts,and could be expanded by supplementation with epidermal growth factor.Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers,neurofilament-200 and NeuN,in vitro.Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice.At 8 weeks,cells positive for LacZ and β-galactosidase staining were observed in the ischemic tissues,and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining.These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.

Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL / 6J mice were separated and cultured using the “pour-off method”. Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor / epidermal growth factor / nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL / 6J mice. At 8 weeks, cells positive for LacZ and β -galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. these findings suggest that the no n-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.