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目的探讨乙酰肝素酶在食管鳞状细胞癌(ESCC)组织中的表达及其与ESCC临床病理特征间的关系。方法应用RT-PCR和免疫组化方法检测55例ESCC术后组织标本及相应癌旁组织中乙酰肝素酶的表达,分析乙酰肝素酶表达与ESCC临床病理特征的关系。结果ESCC组织中存在乙酰肝素酶基因的表达,并定位于肿瘤细胞质和(或)细胞膜中。55例ESCC组织中有40例乙酰肝素酶基因mRNA表达阳性(40/55,72.7%),癌旁组织有19例表达阳性(19/55,34.5%),两组间差异有显著性(P<0.01)。免疫组化染色ESCC组织中乙酰肝素酶阳性率63.6%(35/55),癌旁组织阳性率21.8%(12/55),两组间差异有显著性(P<0.01)。乙酰肝素酶基因mRNA和蛋白质的检测均显示有淋巴结转移的癌组织乙酰肝素酶表达高于无淋巴结转移组,TNM分期Ⅲ~Ⅳa的癌组织表达高于Ⅰ~Ⅱ组,均有统计学意义。结论ESCC组织乙酰肝素酶基因及蛋白表达高于正常组织,并且与ESCC的转移和进展有密切关系。
Objective To investigate the expression of heparanase in esophageal squamous cell carcinoma (ESCC) and its relationship with clinicopathological features of ESCC. Methods The expression of heparanase in 55 specimens of esophageal squamous cell carcinoma (ESCC) and corresponding paracancerous tissues was detected by RT-PCR and immunohistochemistry. The relationship between the expression of heparanase and clinicopathological features of ESCC was analyzed. Results The heparanase gene was expressed in ESCC tissues and localized in the cytoplasm and / or cell membrane of the tumor. Among 55 cases of ESCC, 40 cases had heparanase gene mRNA expression (40/55, 72.7%) and 19 cases (19.55%, 34.5%), there was significant difference between the two groups P <0.01). The positive rate of heparanase in ESCC was 63.6% (35/55) and that in paracancerous tissue was 21.8% (12/55) in immunohistochemical staining. There was significant difference between the two groups (P <0.01). Detection of heparanase mRNA and protein showed that the expression of heparanase in cancer tissues with lymph node metastasis was higher than that without lymph node metastasis, and the TNM stage Ⅲ ~ Ⅳa cancer tissues were higher than those in Ⅰ ~ Ⅱ group significance. Conclusion The expression of heparanase gene and protein in ESCC tissues is higher than that in normal tissues, and is closely related to the metastasis and progression of ESCC.