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构建了线性化的、从基本核心启动子（Ｃｐ）开始并有不同长度Ｃｐ上游顺序的、含完整转录单元的ＨＢＶ基因组克隆，以研究Ｃｐ及其上游顺序对ＨＢＶ基因表达的调控及对ＨＢＶ子代ＤＮＡ复制的影响。发现从基本核心启动子Ｃｐ开始的ＨＢＶ转录单元能够有效地起始３．５ｋｂｍＲ－ＮＡ转录。Ｃｐ上游ＥＮＩ顺序对子代ＤＮＡ复制有极强的刺激作用，而ＥＮⅡ更上游顺序对ＥＮⅡ的刺激作用又有所抑制，证明Ｃｐ的转录受其上游顺序的正负双重调控。另外，Ｃｐ上游还存在与ＨＢＶ基因表达的细胞专一性有关的调控顺序。
A linearized HBV genomic clone containing the complete transcriptional unit, starting from the basic core promoter (Cp) and having a different upstream sequence of Cp, was constructed to investigate the regulation of HBV gene expression by Cp and its upstream sequence, Impact of DNA replication. The HBV transcription unit starting from the basic core promoter Cp was found to be able to efficiently initiate 3.5 kbmR-NA transcription. The upstream ENI sequence of Cp has a strong stimulating effect on the DNA replication of the offspring, whereas the more upstream EN II inhibits the stimulation of ENII, indicating that the transcription of Cp is double-regulated by its upstream and downstream sequences. In addition, there are regulatory sequences upstream of Cp that are involved in cell-specificity of HBV gene expression.