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目的采用PCR技术对ABO血型系统进行基因型检验。方法选择最佳扩增条件进行四引物复合扩增,用限制性内切酶KpnI和AluI分别酶解扩增产物,电泳分离、银染显色法检验ABO基因型。结果对270例血斑、20例混合斑、20根毛发(有毛囊)、12份唾液斑等不同的生物检材进行了分型,与血清学方法检验结果相符。结论该方法能够应用于法医学的检验
Objective To detect genotypes of ABO blood group using PCR technique. Methods The optimal amplification conditions were selected for four-primer amplification. The amplified products were digested with KpnI and AluI respectively. The ABO genotypes were detected by silver staining. Results A total of 270 blood spots, 20 mixed spots, 20 hair follicles, 12 salivary spots were collected and classified according to the results of serological test. Conclusion The method can be applied to the forensic test