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目的探讨AnnexinⅠ蛋白在胰腺癌组织中的表达其临床意义。方法采用免疫组织化学及免疫印迹方法检测40例胰腺癌组织及其相应的癌旁组织、20例正常胰腺组织中AnnexinⅠ蛋白的表达,并分析胰腺癌组织中AnnexinⅠ蛋白的表达与临床病理特征尤其是病理分级、临床分期及转移的关系。结果胰腺癌AnnexinⅠ蛋白阳性表达率为72.5%,癌旁组织为37.5%,正常胰腺组织为20%,三者呈递减趋势,各组之间差异有统计学意义(P<0.05)。胰腺癌AnnexinⅠ蛋白的表达与肿瘤分化程度、TNM分期、淋巴结转移有关。Western blot检测结果与免疫检测方法结果一致。结论 AnnexinⅠ在胰腺癌组织中高表达,且其表达与肿瘤分级有关,提示AnnexinⅠ可能通过免疫机制参与胰腺癌的发生、发展过程。
Objective To investigate the clinical significance of Annexin Ⅰ protein expression in pancreatic cancer. Methods Immunohistochemistry and Western blotting were used to detect the expression of Annexin Ⅰ protein in 40 cases of pancreatic cancer and its corresponding paracancerous tissues and 20 cases of normal pancreatic tissues. The expression of Annexin Ⅰ protein in pancreatic cancer tissues and the clinicopathological features were analyzed Pathological grade, clinical stage and metastasis. Results The positive rates of Annexin Ⅰ protein in pancreatic cancer were 72.5%, 37.5% in paracancerous tissues and 20% in normal pancreatic tissues. The three showed a decreasing trend. The difference between the two groups was statistically significant (P <0.05). The expression of Annexin I protein in pancreatic cancer was correlated with tumor differentiation, TNM stage and lymph node metastasis. Western blot results are consistent with the results of immunoassay. Conclusions Annexin Ⅰ is overexpressed in pancreatic cancer tissues, and its expression is correlated with tumor grade. It suggests that Annexin Ⅰ may play an important role in the pathogenesis and progression of pancreatic cancer through immune mechanism.