论文部分内容阅读
目的：建立双黄连口服液中黄芩苷和绿原酸含量测定的毛细管电泳法。方法：用毛细管区带电泳和紫外检测模式测定双黄连口服液中黄芩苷和绿原酸的含量，电泳条件：以４０ ｍ ｍｏｌ·Ｌ－ １ 硼砂为电泳介质（ 测定黄芩苷和绿原酸的ｐＨ值分别为９－００ 和９－５５） ，未涂层弹性融硅毛细管（５０ μｍ ×３９－５ ｃｍ ，有效分离长度３４－８ ｃｍ） 为分离通道，压力进样（６８－９５ ｋＰａ·ｓ），１７ ｋＶ恒压电泳（２５ ℃） ，黄芩苷和绿原酸的检测波长分别为２８５ ，３２６ ｎｍ 。结果：在２０～６４０μｇ·ｍＬ－１ 和８ ～４００ μｇ·ｍＬ－１ 范围内，黄芩苷和绿原酸可分别进行定量分析，二者加样回收率分别为１００－６０ ％ ±２－３６ ％ 和９９－６８％ ±２－２７ ％ 。结论：本方法简便、快速，结果准确，重现性好，可用于双黄连口服液的质量控制
Objective: To establish a capillary electrophoresis method for the determination of baicalin and chlorogenic acid in Shuanghuanglian oral liquid. Methods: The contents of baicalin and chlorogenic acid in Shuanghuanglian oral liquid were determined by capillary zone electrophoresis and ultraviolet detection. The electrophoresis conditions were: 40 m mol·L-1 borax as the electrophoretic medium (assay for determination of baicalin and chlorogenic acid The pH values were 9-00 and 9-55, respectively, and the uncoated elastic silicon melt capillary (50 μm × 39-5 cm, effective separation length 34-8 cm) was the separation channel, pressure injection (68-95 kPa· s), 17 kV constant pressure electrophoresis (25 °C), baicalin and chlorogenic acid detection wavelength were 285, 326 nm. Results: In the range of 20-640 μg·mL-1 and 8-400 μg·mL-1, baicalin and chlorogenic acid could be quantitatively analyzed, respectively. The recoveries of the two samples were 100-60 %±2-36 respectively. % and 99-68% ± 2-27%. Conclusion: This method is simple, rapid, accurate and reproducible. It can be used for the quality control of Shuanghuanglian Oral Liquid.