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目的在乙酸诱导的大鼠结肠炎模型中,研究普伐他汀的治疗作用及其抗氧化损伤作用的机制。方法SD大鼠60只,随机分为正常对照组(10只)、模型安慰剂组(20只)、普伐他汀治疗组(15只)、柳氮磺吡啶(SASP)治疗组(15只)。正常组常规饲养,模型安慰剂组、普伐他汀治疗组、SASP治疗组8%乙酸造模后分别予以生理盐水2ml/d、普伐他汀1mg·kg-1·d-1、SASP0.25g·kg-1·d-1灌胃治疗7d,观察大鼠活动状态,进食量,体重,大便性状,大便出血情况,计算疾病活动指数(DAI),判断疗效。第8天处死大鼠并进行结肠长度测量,结肠黏膜损伤指数(CMDI)评分,组织学评级,测定组织一氧化氮(NO)自由基、脂质过氧化产物丙二醛(MDA)以及超氧化物歧化酶(SOD)含量。结果与模型安慰剂组及SASP治疗组相比,普伐他汀治疗组DAI、结肠长度、CMDI、组织学评级均有显著改善(P<0.05),NO自由基、MDA含量显著下降,SOD含量显著提高(P<0.05)。结论普伐他汀可通过抑制氧化损伤减轻结肠炎症损伤,且疗效优于传统药物SASP。
Objective To investigate the therapeutic effect of pravastatin and its anti-oxidative mechanism in acetic-induced rat colitis model. Methods Sixty SD rats were randomly divided into normal control group (n = 10), model placebo group (n = 20), pravastatin treatment group (n = 15) and sulfasalazine treatment group . The rats in normal group, model placebo group, pravastatin treatment group and SASP treatment group were given normal saline 2ml / d, pravastatin 1mg · kg-1 · d-1 and SASP 0.25g · kg-1 · d-1 for 7 days. The activity status, food intake, body weight, stool characteristics and stool bleeding were observed. The disease activity index (DAI) was calculated to evaluate the therapeutic effect. On the 8th day, the rats were sacrificed and the length of the colon was measured, the colonic mucosal injury index (CMDI) score, the histological grade, the content of nitric oxide (NO), the malondialdehyde (MDA) Dismutase (SOD) content. Results Compared with the placebo group and the SASP treatment group, the DAI, colon length, CMDI and histological grading of pravastatin group were significantly improved (P <0.05), the contents of NO, MDA and MDA were significantly decreased Increase (P <0.05). Conclusion Pravastatin can reduce colonic inflammatory injury by inhibiting oxidative damage, and its efficacy is better than that of traditional drug SASP.