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目的：检测抑癌基因ｐ１６蛋白在７５例肺癌组织中的表达，并与癌旁组织和正常肺组织对比。结合肺癌的病理特征及预后进行分析，探讨ｐ１６基因与肺癌发生、发展的关系。方法：应用免疫组化ＳＡＢＣ法。结果：正常肺组织、癌旁组织和肺癌组织中ｐ１６蛋白阳性表达率分别为９１．７％、７５．０％和４９．３％，不同类型肺癌组织中ｐ１６蛋白的表达不同（Ｐ＜０．０５）；随着肿瘤分化程度的降低，ｐ１６蛋白的表达下降（Ｐ＜０．０５）；Ⅰ期和Ⅱ期肺癌组织中ｐ１６蛋白阳性表达率明显高于Ⅲ期和Ⅳ期（Ｐ＜０．０５）。生存３年以上肺癌ｐ１６蛋白阳性表达率明显高于生存３年以下者（Ｐ＜０．０５）。结论：ｐ１６基因缺失与肺癌发生有关，不同类型肺癌发生中ｐ１６基因的作用不同，ｐ１６蛋白表达缺失是肺癌的晚期表现，可作为判断肺癌生物学行为和预后的参考指标。
OBJECTIVE: To detect the expression of tumor suppressor gene p16 protein in 75 cases of lung cancer, and compare it with adjacent tissues and normal lung tissues. Combined with the pathological features and prognosis of lung cancer, the relationship between p16 gene and the occurrence and development of lung cancer was discussed. Methods: The application of immunohistochemical SABC method. Results: The positive expression rate of p16 protein in normal lung tissue, paracancerous tissue, and lung cancer tissue were 91.7%, 75.0%, and 49.3%, respectively. The expression of p16 protein was different in different types of lung cancer tissues (P<0. 05); With the decrease of tumor differentiation, the expression of p16 protein decreased (P<0.05); the positive expression rate of p16 protein in stage I and II lung cancer tissues was significantly higher than that of stage III and IV (P<0. 05). The positive expression rate of p16 protein in lung cancer was more than 3 years after survival (P<0.05). Conclusion: The deletion of p16 gene is related to the occurrence of lung cancer. The p16 gene plays a different role in the development of different types of lung cancer. The loss of p16 protein expression is the late stage of lung cancer. It can be used as a reference index to judge the biological behavior and prognosis of lung cancer.