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目的探讨血管内皮生长因子(VEGF)对食管癌血管生成的影响。方法应用SP法对90例食管鳞癌组织和34例癌旁正常黏膜进行VEGF和CD34免疫组化染色,检测VEGF表达和微血管密度(MVD),分析VEGF的表达、MVD以及食管癌临床病理特征之间的关系。结果食管癌组织中VEGF的阳性表达率和MVD分别为71.1%和29.70±3.82,显著高于癌旁正常黏膜的11.76%和15.10±2.38(P<0.01)。VEGF表达及MVD均值与淋巴结转移、肿瘤TNM分期有关(P<0.05和P<0.01),与患者年龄、性别、分化程度、以及肿瘤组织学类型、肿瘤部位均无关(P>0.05);VEGF表达与MVD均值呈显著正相关(P<0.01)。结论 VEGF异常表达在食管癌的血管生成中起重要作用,VEGF及其诱导的血管生成与食管癌的侵袭和转移密切相关。
Objective To investigate the effect of vascular endothelial growth factor (VEGF) on angiogenesis in esophageal cancer. Methods 90 cases of esophageal squamous cell carcinoma and 34 cases of adjacent normal mucosa were immunohistochemically stained for VEGF and CD34 using SP method. VEGF expression and microvessel density (MVD) were detected by immunohistochemistry. The expression of VEGF, MVD and the clinicopathological features of esophageal carcinoma Relationship between. Results The positive rate of VEGF and MVD in esophageal cancer tissues were 71.1% and 29.70 ± 3.82, respectively, which were significantly higher than those in normal mucosa adjacent to cancer (11.76% and 15.10 ± 2.38, P <0.01). The expression of VEGF and MVD were correlated with lymph node metastasis and tumor TNM stage (P <0.05 and P <0.01), but not with age, gender, differentiation degree, tumor histological type and tumor location (P> 0.05) There was a significant positive correlation with MVD (P <0.01). Conclusion The abnormal expression of VEGF plays an important role in the angiogenesis of esophageal cancer. The VEGF and its angiogenesis are closely related to the invasion and metastasis of esophageal cancer.