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目的:观察克罗恩病患者中血清中TNF-a的含量,探讨这种炎性因子在该病诊断中所起的作用。方法:采用酶联免疫吸附试验(ELISA法)检测28例已明确诊断为克罗恩病患者和36例体检健康者的血清标本,分析炎性因子TNF-a的含量变化,比较这种炎性因子在克罗恩病患者和健康者的差异。结果:与正常组相比,TNF-a的含量明显升高(P<0.001)。结论:克罗恩病患者存在严重的细胞免疫功能紊乱;TNF-a的含量明显升高,测定炎症因子的变化可以为克罗恩病的诊断提供一定的依据。
Objective: To observe the content of serum TNF-a in Crohn’s disease patients and to explore the role of this inflammatory factor in the diagnosis of the disease. Methods: Serum samples from 28 Crohn’s disease patients and 36 healthy controls were detected by enzyme-linked immunosorbent assay (ELISA), and the changes of inflammatory cytokines TNF-a were analyzed. Differences in factors between Crohn’s disease and healthy subjects. Results: Compared with the normal group, the content of TNF-a was significantly increased (P <0.001). CONCLUSIONS: Patients with Crohn’s disease have severe cellular immune dysfunction and TNF-a levels are significantly increased. Measuring the changes of inflammatory cytokines may provide a basis for the diagnosis of Crohn’s disease.