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目的探讨超声破坏卵巢癌靶向超声造影剂(ovarian cancer targeted ultrasound contrast agent,OCTUCA)即黏附促黄体生成激素释放激素(luteinizing hormone releasing hormone,LHRH)的脂质体微泡(LM)联合紫杉醇对人卵巢癌OVCAR3细胞株增殖、凋亡及细胞内药物浓度的影响。方法在体外培养OVCAR3细胞株,经MTT比色法检测紫杉醇对OVCAR3体外治疗的有效浓度,然后分别用OCTUCA、紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉醇及超声破坏OCTUCA联合紫杉醇进行处理,MTT检测细胞增殖抑制率、流式细胞技术(FCM)检测细胞凋亡率、高效液相色谱(HPLC)法检测细胞内紫杉醇浓度。结果紫杉醇对OVCAR3体外治疗的有效浓度是10-6mol/L,超声破坏OCTUCA联合紫杉醇组细胞增殖抑制率及凋亡率均明显高于OCTUCA、紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉处理组(P均<0.05),超声破坏OTUCA联合紫杉醇组细胞内药物浓度明显高于紫杉醇、紫杉醇联合超声、超声破坏脂质体微泡联合紫杉醇处理组(P<0.01)。结论超声破坏OCTUCA联合紫杉醇能使OVCAR3细胞内紫杉醇药物浓度增加,抑制其增殖,并诱导其凋亡。
OBJECTIVE: To investigate the effect of liposomal microbubbles (LM) combined with paclitaxel on the destruction of ovarian cancer targeted ultrasound contrast agent (OCTUCA), ie, luteinizing hormone releasing hormone (LHRH) Effect of ovarian cancer OVCAR3 cell proliferation, apoptosis and intracellular drug concentration. Methods OVCAR3 cells were cultured in vitro and the effective concentration of paclitaxel on OVCAR3 in vitro was determined by MTT colorimetric assay. Then OCTUCA, paclitaxel, paclitaxel combined with ultrasound, ultrasound-induced liposome microbubbles combined with paclitaxel and ultrasound were used to destroy OCTUCA and paclitaxel The inhibitory rate of cell proliferation was detected by MTT, the apoptosis rate was detected by flow cytometry (FCM) and the paclitaxel concentration was detected by high performance liquid chromatography (HPLC). Results The effective concentration of paclitaxel for OVCAR3 in vitro treatment was 10-6mol / L. The inhibition rate and apoptosis rate of OCTUCA combined with paclitaxel group were significantly higher than those of OCTUCA, paclitaxel, paclitaxel combined with ultrasound and liposome microbubbles combined (P <0.05). The intracellular drug concentration in the combination of paclitaxel and paclitaxel was significantly higher than paclitaxel, paclitaxel combined with ultrasound and liposome microbubbles combined with paclitaxel treatment (P <0.01). Conclusion Ultrasound-induced OCTUCA combined with paclitaxel can increase paclitaxel concentration in OVCAR3 cells, inhibit its proliferation and induce its apoptosis.