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目的利用宫颈癌细胞株探讨血根碱(SAN)的抗肿瘤作用。方法采用MTT、克隆形成及细胞划痕试验研究不同浓度SAN对体外培养的人类宫颈癌HeLa和Siha细胞的影响。结果经1.0μmol/L和5.0μmol/LSAN处理后,Siha细胞的存活率分别为对照组的72%和10%(P<0.01),HeLa细胞比Siha细胞更敏感。用1.0μmol/LSAN处理后,HeLa和Siha细胞的克隆形成数从33个降低到14个和16个(P<0.05)。划痕试验显示培养48h后,HeLa细胞和Siha细胞的“细胞伤口”宽度对照组分别为(0.51±0.04)mm和(0.64±0.02)mm,而0.5μmol/LSAN处理组为(1.22±0.02)mm和(1.63±0.01)mm(分别P<0.01和P<0.05)。MTT、克隆形成及细胞划痕试验均呈剂量和时间依赖性。结论SAN可抑制宫颈癌细胞的生长和转移。该研究为SAN作为一种新药应用于宫颈癌的治疗提供了初步的实验依据和理论基础。
Objective To investigate the antitumor effects of sanguinarine (SAN) using cervical cancer cell lines. Methods The effects of different concentrations of SAN on human cervical carcinoma HeLa and Siha cells cultured in vitro were studied using MTT assay, colony formation assay and cell scratch assay. Results After treatment with 1.0μmol/L and 5.0μmol/L SAN, the survival rate of Siha cells was 72% and 10% of the control group, respectively (P<0.01). HeLa cells were more sensitive than Siha cells. After treatment with 1.0 μmol/L SAN, the number of colony formation of HeLa and Siha cells decreased from 33 to 14 and 16 (P<0.05). The scratch test showed that the HeLa cells and Siha cells had a “wound width” control group of (0.51 ± 0.04) mm and (0.64 ± 0.02) mm, respectively, and a 0.5 μmol/L SAN treatment group of (1.22 ± 0.4%). 0.02) mm and (1.63±0.01) mm (P<0.01 and P<0.05, respectively). MTT, colony formation, and cell scratch assays were dose- and time-dependent. Conclusion SAN can inhibit the growth and metastasis of cervical cancer cells. The study provides a preliminary experimental basis and theoretical basis for SAN as a new drug for the treatment of cervical cancer.