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为了进一步研究人活化素结合蛋白的免疫生物学活性,从人卵泡液中提取了活化素结合蛋白,采用亲和层析及高效液相层析法,最终获得35KDa,SDS-PAGE显示为一条带的人活化素结合蛋白,经免疫活性测定、激活素结合试验以及FSH分泌抑制试验证实,所纯化的人活化素结合蛋白为具有生物学活性的天然活化素结合蛋白,可供进一步的免疫生物学研究用。
In order to further study the immunobiological activity of human activin-binding protein, activin-binding protein was extracted from human follicular fluid and finally 35 KDa was obtained by affinity chromatography and high performance liquid chromatography. SDS-PAGE showed a band Of human activin binding protein, confirmed by immunoactivity assay, activin binding assay and FSH secretion inhibition test, the purified human activin binding protein is a biologically active natural activin-binding protein that can be used for further immunobiology Research use.