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胆道闭锁是以肝内外胆管进行性炎症和纤维化为特征的疾病，是导致婴儿持续性黄疸的重要原因，如未得到及时治疗，可迅速发展为肝硬化，患儿最终因肝功能衰竭而死亡，因此胆道闭锁的早期诊断显得尤为重要。胆道闭锁主要依靠术中胆道造影以及肝组织活检确诊，但其具有侵入性、操作复杂、并发症多等缺点，不利于胆道闭锁的早期诊断，相比较而言，一些无创性检查方法如实验室检查、影像学检查等具有明显优势。本文对各种胆道闭锁诊断方法的应用价值进行分析，为其早期诊断提供新思路。“,”Biliary atresia is a disease characterized by progressive inflammation and fibrosis of intrahepatic and extrahepatic bile ducts, which is an important cause of persistent jaundice in infants, Without timely treatment, it can develop rapidly into cirrhosis, and the child will die of liver failure. Therefore, the early diagnosis of biliary atresia is particularly important. The diagnosis of biliary atresia mainly depends on intraoperative cholangiography and liver biopsy, but they have the disadvantages including invasion, complex operation and many complications, which is not conducive to the early diagnosis of biliary atresia. In comparison, some non-invasive examination methods such as laboratory examination and imaging examination have obvious advantages. In this paper, the value of various diagnostic methods of biliary atresia is analyzed, which provides new ideas for its early diagnosis.