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作者调查了世界上胃癌发病率最高的日本人的膳食,发现鱼是其膳食中的关键组分。而在波兰和其它东欧国家猪肉、豆、大麦、酸奶、布什汤(borscht)消耗很多,胃癌发病率同样也高。美国食用红肠和牛肉较多,胃癌发病率低。作者对上述食品与胃癌的关系进行了研究。将上述食品磨匀后,取5g,加入含5,000ppm NaNO_2和20,000ppm NaCl 的溶液1ml。对照样品用1ml 蒸馏水代替。于暗处25℃、pH3、条件下培养1小时,然后以乙醚提取、过滤、浓缩得到亚硝酸盐处理后的食品提取液。用鼠伤寒沙门氏菌作指示菌进行提取液的致突变试验。试验结果说明,经亚硝酸盐处理过的鱼的
The authors investigated the Japanese diet, which had the highest rates of gastric cancer in the world, and found that fish was a key component of its diet. In Poland and other eastern European countries pork, beans, barley, yogurt, and borscht consume a lot, and the incidence of gastric cancer is also high. The United States eat more sausage and beef, the incidence of gastric cancer is low. The authors studied the relationship between these foods and gastric cancer. After grinding the above food uniformly, take 5 g and add 1 ml of a solution containing 5,000 ppm NaNO 2 and 20,000 ppm NaCl. The control sample was replaced with 1 ml of distilled water. In the dark at 25 ℃, pH3, conditions for 1 hour, then extracted with ether, filtered and concentrated to give nitrite treated food extract. Salmonella typhimurium as indicator bacteria for mutagenicity test. The test results show that the nitrite-treated fish