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目的对分离自河北省长爪沙鼠鼠疫疫源地的116株鼠疫菌株进行基因分析研究。方法根据鼠疫基因组比对,选择15对引物对测试菌株进行PCR扩增,并对扩增结果进行分析。结果 15对引物对116株实验菌株均得到相应的扩增结果,同一引物测试菌株扩增结果目标条带长度均一致,串联重复序列的重复数相同。不同引物扩增目标条带长度不同,串联重复序列的重复数也不同,如引物M52对所有菌株扩增产物长度均为153 bp,串联重复序列的重复数均为3,而引物M59的扩增产物长度为250 bp,重复数均为7。结论多位点串联重复序列分析(MLVA)证实河北省鼠疫自然疫源地的鼠疫菌基因稳定,鼠疫菌MLVA数据库将对未来的鼠疫菌变异和溯源提供技术支持。
Objective To analyze the genetic distribution of 116 Y. pestis isolates isolated from the gerbils of Gekko gecko in Hebei province. Methods According to the plague genome comparison, 15 pairs of primers were selected for PCR amplification of the tested strains, and the amplification results were analyzed. Results All the 116 pairs of primer pairs obtained the corresponding amplification results. The length of target bands amplified by the same primers was consistent, and the number of tandem repeats was the same. The lengths of the target bands amplified by different primers are different, and the number of tandem repeats is also different. For example, the length of all amplified products of primer M52 is 153 bp and the number of tandem repeats is 3, while the amplification of primer M59 The product length is 250 bp and the number of repeats is 7. Conclusions Multilocus tandem repeat analysis (MLVA) confirmed the stability of plague genes in the plague natural foci of Hebei Province. The plague MLVA database will provide technical support for future Yersinia pestis variation and traceability.