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作者曾报道有关给NMRI小鼠去核艾氏腹水癌细胞(细胞质)活性免疫。给细胞质后20天,动物对肿瘤完全有抵抗力。本文进一步报道,实验鼠给NMRI小鼠胚胎匀浆后,对艾氏腹水癌细胞也有抵抗力。按Schone的实验方法,用超速匀浆器(2000 U/分)将NMRI小鼠胚胎(胚胎/ml0.9%无菌生理盐水,胚胎大小未经选择)研成匀浆,置于4°玻璃匀浆器内(5ml)再研3分钟,然后用超声波60s(Blanson SonifierS75,4级,电流强度45A)处理。小鼠腹腔注射0.8ml匀浆。7天后再给一次匀浆,再过7天后产生免疫。
The authors have reported on the activity of nuclear echinuclear cancer cells (cytoplasm) immunoreactivity to NMRI mice. Twenty days after the cytoplasm, the animal is completely resistant to the tumor. This article further reports, experimental mice to NMRI mouse embryos homogenate, Ehrlich ascites cancer cells are also resistant. NMRI mouse embryos (embryos / ml 0.9% sterile saline, embryo size unselected) were homogenized using a speeding homogenizer (2000 U / min) according to Schone’s protocol and placed in a 4 ° glass The inside of the homogenizer (5 ml) was further pulverized for 3 minutes and then treated with ultrasonic waves for 60 s (Grade 4, 4, current 45 A). Mice were injected intraperitoneally 0.8ml homogenate. After 7 days to give a homogenate, another 7 days after the immune.