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目的:探究孕激素对人蜕膜基质细胞体外增殖、凋亡的影响。方法:原代培养蜕膜细胞,采用四甲基偶氮咗蓝(MTT)比色法及流式细胞仪检测孕酮对细胞增殖、凋亡的影响。结果:20~80μmol/L孕酮可抑制蜕膜细胞的增殖活力(P<0.05),可引起蜕膜细胞形态变化。10~80μmol/L孕酮可提高蜕膜细胞总凋亡率(P<0.05),但其总凋亡率仍低于6%。结论:孕酮可以抑制人早孕子宫蜕膜细胞的增殖,作用48 h时抑制率与孕酮浓度正相关;孕酮可诱导蜕膜细胞凋亡。
Objective: To investigate the effects of progesterone on proliferation and apoptosis of human decidual stromal cells in vitro. Methods: The primary cultured human decidual cells were cultured in vitro. The effects of progesterone on proliferation and apoptosis were detected by MTT assay and flow cytometry. Results: Progesterone (20-80 μmol / L) could inhibit the proliferation of decidual cells (P <0.05), which could cause morphological changes of decidual cells. Progesterone at 10 ~ 80μmol / L increased the total apoptosis rate of decidual cells (P <0.05), but the total apoptosis rate was still below 6%. CONCLUSION: Progesterone can inhibit the proliferation of human uterine decidual cells in early pregnancy. The inhibitory rate at 48 h is positively correlated with the concentration of progesterone. Progesterone can induce the apoptosis of decidual cells.