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为研究临床诊断为非乙非丙型慢性肝炎患者中的庚型肝炎病毒感染，应用逆转录套式多聚酶链反应法检测其血清中的ＨＧＶ／ＧＢＶＣＲＮＡ和ＨＣＶＲＮＡ，并用多聚酶链反应检测ＨＢＶＤＮＡ。采用合成肽抗原作为固相，以ＥＩＡ法检测抗ＧＢＶＣ抗体；应用ＨＣＶ不同功能区抗原，分片段检测抗ＨＣＶ抗体。结果表明，临床诊断为非乙非丙慢性肝炎患者血清中，１．０４％（１／９６）为ＨＢＶＤＮＡ阳性；１０．４２％（１０／９６）为ＨＣＶＲＮＡ或抗ＨＣＶ阳性；２７．０８％（２６／９６）为抗ＨＧＶ／ＧＢＶＣ阳性或ＨＧＶ／ＧＢＶＣＲＮＡ阳性。研究提示庚型肝炎病毒是临床非乙非丙型慢性肝炎的重要致病因子；还可能存在非乙非丙非庚型慢性肝炎的致病因子；ＨＣＶ感染的检测水平有必要进一步提高。
To study the hepatitis G virus infection in patients with clinically diagnosed non-B non-B hepatitis, HGV / GBVCRNA and HCVRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) and HBVDNA was detected by polymerase chain reaction. Using synthetic peptide antigen as the solid phase to EIA anti-GBV C antibody; application of different functional areas of HCV antigen, sub-fragment detection of anti-HCV antibodies. The results showed that 1.04% (1/96) of the sera were clinically diagnosed as non-HBeAg-positive hepatitis B virus (HBVDNA) positive, 10.42% (10/96) were HCVRNA or anti-HCV, 27.08% 26/96) for anti- GHV / GBV C positive or HGV / GBV CRNA positive. Studies have shown that hepatitis G virus is an important virulence factor in non-B hepatitis C non-B hepatitis; there may be a risk factor for non-B non-HepG chronic hepatitis; HCV infection levels need to be further improved.