论文部分内容阅读
目的:探讨无花果叶提取物对OPG小鼠的抗骨质疏松作用及机制。方法:8周龄OPG基因敲除雌性小鼠60只,随机分成:实验组(无花果叶提取物组)、对照组(福善美组)、空白组(0.9%氯化钠溶液组),分别于喂药30、60d时用HE染色方法观察各组间骨小梁变化;用免疫组化法研究骨组织中白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)的定位及信号表达强弱。结果:灌胃60d后实验组、对照组与空白组比较,骨小梁数目增加,差异有统计学意义(P<0.05);灌胃60d后实验组、对照组与空白组比较,IL-1和TNF-α阳性表达的细胞数目减少(P<0.05)。结论:无花果叶提取物具有抗骨质疏松作用,此作用可能与降低OPG基因敲除小鼠骨组织中IL-1、TNF-α的阳性细胞表达有关。
Objective: To investigate the anti-osteoporosis effect of fig leaf extract on OPG mice and its mechanism. Methods: Sixty female OPG knockout mice of 8 weeks old were randomly divided into experimental group (fig leaf extract group), control group (FuShanMei group) and blank group (0.9% sodium chloride solution group) At 30 and 60 days after feeding, the changes of trabecular bone were observed by HE staining. The location of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) Signal expression strength. Results: Compared with the blank control group, the number of trabecular bone increased in the control group and the control group after 60 days of gavage (P <0.05). Compared with the blank control group, the levels of IL-1 And the number of TNF-a positive cells decreased (P <0.05). CONCLUSION: Fig leaf extract has anti-osteoporosis effect, which may be related to the decrease of the expression of IL-1 and TNF-α positive cells in OPG knockout mice bone.